• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MBD1 Polyklonaler Antikörper

MBD1 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29998-1-PBS

Synonyme

MBD 1

Filter:
  • Western Blot (WB) analysis of various lysates using MBD1 Polyclonal antibody (29998-1-AP)

    WB analysis using 29998-1-AP (same clone as 29998-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29998-1-AP (MBD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29998-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MBD1 Polyclonal antibody (29998-1-AP)

    IF Staining of HeLa using 29998-1-AP (same clone as 29998-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MBD1 antibody (29998-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 29998-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MBD1 Polyclonal antibody (29998-1-AP)

    IF Staining of MCF-7 using 29998-1-AP (same clone as 29998-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MBD1 antibody (29998-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 29998-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

29998-1-PBS bindet in WB, IF/ICC, Indirect ELISA MBD1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MBD1 fusion protein Ag31424
Vollständiger Name methyl-CpG binding domain protein 1
Berechnetes Molekulargewicht67KD
Beobachtetes Molekulargewicht70-80 kDa
GenBank-ZugangsnummerNM_015846
Gene symbol MBD1
Gene ID (NCBI) 4152
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MBD1, also known as RFT, PCM1, CXXC3, is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). MBD1 is a transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD1 acts as a transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. Isoform 1 and isoform 2 can repress transcription from unmethylated promoters (PMID: 12665582, 14610093, 16757475).