MBD2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MBD2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 55200-1-AP

Synonyme

Demethylase, DMTase, MBD2, NY CO 41

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 55200-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55200-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55200-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55200-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55200-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55200-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55200-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55200-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 55200-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55200-1-AP (MBD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 55200-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55200-1-AP (MBD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A431-Zellen, HEK-293-Zellen, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

55200-1-AP bindet in WB, IHC, IF, ELISA MBD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: methyl-CpG binding domain protein 2
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43-45 kDa
• GenBank-Zugangsnummer: NM_003927
• Gene symbol: MBD2
• Gene ID (NCBI): 8932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MBD2, also named as DMTase, binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD2 binds hemi-methylated DNA as well. It recruits histone deacetylases and DNA methyltransferases. MBD2 acts as transcriptional repressor and plays a role in gene silencing. (PMID:9774669) This antibody is specific to MBD2. It recognize isoform1 and isoform2 of MBD2.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang ; KD Validated
mouse	WB	Exp Cell Res; Loss of MBD2 affects early T cell development by inhibiting the WNT signaling pathway.; Authors - Ling Cheng ; KO Validated
human	WB	Cell Rep; TRPS1 drives heterochromatic origin refiring and cancer genome evolution.; Authors - Jianguo Yang
human	WB	Genet Test Mol Biomarkers; The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas.; Authors - Mei-Rong Liang
human	WB,IF	Gene; Exploring the importance of m5c in the diagnosis and subtype classification of COPD using the GEO database; Authors - Jianjun Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Manfred (Verified Customer) (05-09-2022)

The antibody worked perfectly without any unspecific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PBMCs