MBD3 Rekombinanter Antikörper

MBD3 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250922E11

Kat-Nr. : 86681-2-PBS

Synonyme

MBD 3, Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3

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  WB analysis using 86681-2-RR (same clone as 86681-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86681-2-RR (MBD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86681-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86681-2-RR (same clone as 86681-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MBD3 antibody (86681-2-RR, Clone: 250922E11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86681-2-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02598-1

  The mean MBD3 concentration was determined to be 19.2 ng/mL in Y79 cell extract based on a 1.1 mg/mL extract load, 28.9 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 89.6 ng/mL in HEK-293 cell extract based on a 3.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP02598-1

  Cytometric bead array standard curve of MP02598-1, MBD3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86681-3-PBS. Detection antibody: 86681-2-PBS. Standard: Ag5531. Range: 0.781-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

86681-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA MBD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MBD3 fusion protein Ag5531
• Vollständiger Name: methyl-CpG binding domain protein 3
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC043619
• Gene symbol: MBD3
• Gene ID (NCBI): 53615
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Methyl-CpG-binding domain 3 (Mbd3), a member among the five-MBD protein family, is unique in its ability to specifically recognize 5′-hydroxymethyl-cytosine (5′-hmC), an epigenetic marker that is highly enriched in stem cells and cancer.