MBIP Monoklonaler Antikörper

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MBIP Monoklonal Antikörper für WB, IP, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E6H11

Kat-Nr. : 66102-1-PBS

Synonyme

MBIP, MUK binding inhibitory protein

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  WB analysis using 66102-1-Ig (same clone as 66102-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66102-1-Ig (same clone as 66102-1-PBS)

  WB result of MBIP antibody (66102-1-Ig; 1:2000; room temperature for 1.5 hours) with sh-Control and sh-MBIP transfected HEK-293 cells. This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66102-1-Ig (same clone as 66102-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66102-1-Ig (same clone as 66102-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66102-1-Ig (MBIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 66102-1-Ig (same clone as 66102-1-PBS)

  IP result of anti-MBIP (IP:66102-1-Ig, 3ug; Detection:66102-1-Ig 1:300) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66102-1-Ig (same clone as 66102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66102-1-Ig (same clone as 66102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66102-1-Ig (same clone as 66102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66102-1-Ig (same clone as 66102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66102-1-Ig (MBIP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66102-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66102-1-PBS bindet in WB, IP, IHC, Indirect ELISA MBIP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MBIP fusion protein Ag18090
• Vollständiger Name: MAP3K12 binding inhibitory protein 1
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC005197
• Gene symbol: MBIP
• Gene ID (NCBI): 51562
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.