MBL Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MBL Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 24207-1-AP

Synonyme

MBL2, Mannan-binding protein, Mannan binding protein, HSMBPC, Collectin-1

---

• 

  WB analysis of human plasma using 24207-1-AP

  human blood was subjected to SDS PAGE followed by western blot with 24207-1-AP (MBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 24207-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24207-1-AP (MBL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 24207-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24207-1-AP (MBL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Blutgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

24207-1-AP bindet in WB, IHC, IF, ELISA MBL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MBL fusion protein Ag21508
• Vollständiger Name: mannose-binding lectin (protein C) 2, soluble (opsonic defect)
• Berechnetes Molekulargewicht: 248 aa, 26 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC069338
• Gene symbol: MBL2
• Gene ID (NCBI): 4153
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts
human	WB,IHC	Oncol Rep; HIF‑1α and MBP1 are associated with the progression of breast cancer cells by repressing β‑catenin transcription.; Authors - Ying Zhuang
human	WB	medRxiv; Identification of disease-specific extracellular vesicle-associated plasma protein biomarkers for Duchenne Muscular Dystrophy and Facioscapulohumeral Muscular Dystrophy; Authors - Mustafa Bilal Bayazit
human	IF	Adv Sci (Weinh); Exosome-Mediated Lectin Pathway and Resistin-MIF-AA Metabolism Axis Drive Immune Dysfunction in Immune Thrombocytopenia; Authors - Jin Li
human	WB	Cancer Control; MiR-942-3p Promotes the Proliferation and Invasion of Hepatocellular Carcinoma Cells by Targeting MBL2.; Authors - Chun-Yang Xu ; KD Validated
	WB	Biochem Biophys Res Commun; Characterization of a silkworm UFM1 homolog in regulating Bombyx mori unfolded protein response and nucleopolyhedrovirus replication; Authors - Yiting Lu