MBP tag Monoklonaler Antikörper

MBP tag Monoklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

rekombinanten Protein

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

4C6H4

Kat-Nr. : 66003-1-PBS

Synonyme

MBP, Maltose Binding Protein

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  WB analysis of Recombinant protein using 66003-1-Ig (same clone as 66003-1-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 66003-1-Ig (MBP tag antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66003-1-PBS in a different storage buffer formulation.

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  IP experiment of Recombinant protein using 66003-1-Ig (same clone as 66003-1-PBS)

  IP result of anti-MBP tag (IP:66003-1-Ig, 5ug; Detection:66003-1-Ig 1:20000) with Recombinant protein protein lysate 800ug. This data was developed using the same antibody clone with 66003-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected cells using 66003-1-Ig (same clone as 66003-1-PBS)

  IF result of MBP tag antibody (66003-1-Ig, 1:1,000) with MBP-Tagged protein.Courtesy of Neeraj Tiwari, PhD, Yale School of Medicine, Yale University. This data was developed using the same antibody clone with 66003-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66003-1-PBS bindet in WB, IF/ICC, IP, ELISA MBP tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MBP tag fusion protein Ag0942
• Vollständiger Name: MBP tag
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.