MCAM Rekombinanter Antikörper

MCAM Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250530B2

Kat-Nr. : 86492-5-PBS

Synonyme

CD146, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18, S-endo 1 endothelial-associated antigen

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  Sandwich ELISA sample test of MP02466-2

  HeLa were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human CD146/MCAM, and measured 20.8 ng/mL.

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  Sandwich ELISA sample test of MP02466-2

  Plasma of six individual healthy human donors was measured. The CD146/MCAM concentration of detected samples was determined to be 267.2 ng/mL with a range of 157.5-536.7 ng/mL.

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  Sandwich ELISA standard curve of MP02466-2

  Sandwich ELISA standard curve of MP02466-2, Human CD146/MCAM Recombinant Matched Antibody Pair - PBS only. 86492-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2938. 86492-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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Geprüfte Anwendungen

Produktinformation

86492-5-PBS bindet in Sandwich ELISA, Indirect ELISA MCAM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MCAM fusion protein Eg2938
• Vollständiger Name: melanoma cell adhesion molecule
• Berechnetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: NM_006500.2
• Gene symbol: CD146
• Gene ID (NCBI): 4162
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.