MCCC1 Polyklonaler Antikörper

MCCC1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30081-1-PBS

Synonyme

MCCA, MCC B, EC:6.4.1.4, 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha, 3-methylcrotonyl-CoA carboxylase biotin-containing subunit

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  WB analysis using 30081-1-AP (same clone as 30081-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30081-1-AP (MCCC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 30081-1-AP (same clone as 30081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30081-1-AP (MCCC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 30081-1-AP (same clone as 30081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30081-1-AP (MCCC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 30081-1-AP (same clone as 30081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30081-1-AP (MCCC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 30081-1-AP (same clone as 30081-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MCCC1 antibody (30081-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 30081-1-AP (same clone as 30081-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCCC1 antibody (30081-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30081-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30081-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MCCC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MCCC1 fusion protein Ag32459
• Vollständiger Name: methylcrotonoyl-Coenzyme A carboxylase 1 (alpha)
• Berechnetes Molekulargewicht: 76 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC004187
• Gene symbol: MCCC1
• Gene ID (NCBI): 56922
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCCC1 is the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.