MCL1 Monoklonaler Antikörper

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• KD/KO Validated

MCL1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

CloneNo.

1E3C2

Kat-Nr. : 66026-1-Ig

Synonyme

EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM

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  WB analysis using 66026-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66026-1-Ig

  WB result of MCL1 antibody (66026-1-Ig, 1:1000) with si-Control and si-MCL1 transfected Jurkat cells.

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  WB analysis of HeLa using 66026-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 66026-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66026-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66026-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66026-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66026-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66026-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66026-1-Ig (MCL1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66026-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66026-1-Ig (MCL1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66026-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCL1 antibody (66026-1-Ig, Clone: 1E3C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 66026-1-Ig

  Immunofluorescent analysis of Hela cells, using MCL1 antibody 66026-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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  IF Staining of MCF-7 using 66026-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66026-1-Ig (MCL1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, A549-Zellen, Daudi-Zellen, Jurkat-Zellen, Raji-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 19 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

66026-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA MCL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MCL1 fusion protein Ag10609
• Vollständiger Name: myeloid cell leukemia sequence 1 (BCL2-related)
• Berechnetes Molekulargewicht: 350 aa, 37 kDa
• GenBank-Zugangsnummer: BC107735
• Gene symbol: MCL1
• Gene ID (NCBI): 4170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target.

Publikationen

Species	Application	Title
mouse	WB	Int J Mol Sci; Intermittent Fasting Aggravates Lupus Nephritis through Increasing Survival and Autophagy of Antibody Secreting Cells in MRL/lpr Mice.; Authors - Seung-Min Hong
human	WB	Biochim Biophys Acta Mol Basis Dis; Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages; Authors - Ling Zhou
human	WB	Epigenetics; Decreased DHRS2 expression is associated with HDACi resistance and poor prognosis in ovarian cancer.; Authors - Yingyan Han
human	WB	J Nat Prod; Hernandezine Regulates Proliferation and Autophagy-Induced Apoptosis in Melanoma Cells.; Authors - Xiaoting Wang
human	WB	Mol Microbiol; The emerging oral pathogen, Filifactor alocis, extends the functional lifespan of human neutrophils.; Authors - Irina Miralda
human	WB	Ann Transl Med; Adipose-derived stem cells (ADSCs) inhibit the expression of anti-apoptosis proteins through up-regulation of ATF4 on breast cancer cells.; Authors - Jing Ren