MCL1 Rekombinanter Antikörper

MCL1 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5P13

Kat-Nr. : 82695-4-PBS

Synonyme

Induced myeloid leukemia cell differentiation protein Mcl-1, EAT, Bcl-2-related protein EAT/mcl1, Bcl-2-like protein 3, Bcl2-L-3

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  WB analysis using 82695-4-RR (same clone as 82695-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82695-4-RR (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82695-4-RR (same clone as 82695-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82695-4-RR (MCL1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82695-4-RR (same clone as 82695-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82695-4-RR (MCL1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82695-4-RR (same clone as 82695-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82695-4-RR (MCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82695-4-RR (same clone as 82695-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82695-4-RR (MCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 82695-4-RR (same clone as 82695-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCL1 antibody (82695-4-RR, Clone: 5P13 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

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  FC experiment of Ramos using 82695-4-RR (same clone as 82695-4-PBS)

  1x10^6 Ramos cells were intracellularly stained with 0.25 ug MCL1 Recombinant antibody (82695-4-RR, Clone:5P13) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82695-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82695-4-RR against Human MCL1 were performed. The affinity constant is 2.13 nM.

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Geprüfte Anwendungen

Produktinformation

82695-4-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA MCL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MCL1 fusion protein Ag10397
• Vollständiger Name: myeloid cell leukemia sequence 1 (BCL2-related)
• Berechnetes Molekulargewicht: 350 aa, 37 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC107735
• Gene symbol: MCL1
• Gene ID (NCBI): 4170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumor malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target. MCL-1 can be alternatively spliced into a long form (MCL-1L, 40 kDa) or a short form (MCL-1S, 30 kDa). (PMID: 15467463, PMID: 15147382)