MCM10 Polyklonaler Antikörper

MCM10 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Kat-Nr. : 12251-1-AP

Synonyme

CNA43, DNA43, HsMCM10, MCM10, PRO2249, Protein MCM10 homolog

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  WB analysis of Ramos using 12251-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 12251-1-AP (MCM10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IF: See 1 publications below

Produktinformation

12251-1-AP bindet in WB, IF, ELISA MCM10 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MCM10 fusion protein Ag2899
• Vollständiger Name: minichromosome maintenance complex component 10
• Berechnetes Molekulargewicht: 874 aa, 97 kDa
• Beobachtetes Molekulargewicht: 97-105 kDa
• GenBank-Zugangsnummer: BC009108
• Gene symbol: MCM10
• Gene ID (NCBI): 55388
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCM10, also named as HsMCM10, belongs to the MCM10 family. It acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, it plays a role in preventing DNA damage during replication and is essential for the integrity of the RECQ4-MCM replicative helicase complex. It mediates RECQ4 association with MCM2-7 helicase complex during DNA replication.

Publikationen

Species	Application	Title
human		Nat Commun; DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain.; Authors - Jing Zhang
human	WB	Nat Commun; BRCA2 associates with MCM10 to suppress PRIMPOL-mediated repriming and single-stranded gap formation after DNA damage.; Authors - Zhihua Kang
human	WB	Cancer Discov; DDX5 regulates DNA replication and is required for cell proliferation in a subset of breast cancer cells.; Authors - Mazurek Anthony A
human	WB	EMBO J; MCM10 mediates RECQ4 association with MCM2-7 helicase complex during DNA replication.; Authors - Xu Xiaohua X
human		J Cell Biol; Regulation of ETAA1-mediated ATR activation couples DNA replication fidelity and genome stability.; Authors - Divya Achuthankutty
human	WB	Cell Rep; Impaired p32 Regulation Caused by the Lymphoma-Prone RECQ4 Mutation Drives Mitochondrial Dysfunction.; Authors - Jiin-Tarng Wang