MCM2 Polyklonaler Antikörper

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• KD/KO Validated

MCM2 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10513-1-PBS

Synonyme

BM28, CCNL1, CDCL1, EC:3.6.4.12, KIAA0030

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  WB analysis using 10513-1-AP (same clone as 10513-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10513-1-AP (MCM2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10513-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10513-1-AP (same clone as 10513-1-PBS)

  WB result of MCM2 antibody (10513-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM2 transfected HEK-293 cells. This data was developed using the same antibody clone with 10513-1-PBS in a different storage buffer formulation.

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  WB analysis using 10513-1-AP (same clone as 10513-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 10513-1-AP (MCM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10513-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 10513-1-AP (same clone as 10513-1-PBS)

  IP result of anti-MCM2 (IP:10513-1-AP, 4ug; Detection:10513-1-AP 1:800) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 10513-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 10513-1-AP (same clone as 10513-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10513-1-AP (MCM2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10513-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 10513-1-AP (same clone as 10513-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10513-1-AP (MCM2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10513-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 10513-1-AP (same clone as 10513-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10513-1-AP (MCM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10513-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 10513-1-AP (same clone as 10513-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10513-1-AP (MCM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10513-1-PBS in a different storage buffer formulation.

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  IF Staining of human gliomas using 10513-1-AP (same clone as 10513-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using 10513-1-AP (MCM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10513-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10513-1-AP (same clone as 10513-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM2 antibody (10513-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10513-1-PBS in a different storage buffer formulation.

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  FC experiment of NIH/3T3 using 10513-1-AP (same clone as 10513-1-PBS)

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human MCM2 (10513-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 10513-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10513-1-PBS bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA MCM2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MCM2 fusion protein Ag0798
• Vollständiger Name: minichromosome maintenance complex component 2
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 116-125 kDa
• GenBank-Zugangsnummer: BC007670
• Gene symbol: MCM2
• Gene ID (NCBI): 4171
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.

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