MCM2 Monoklonaler Antikörper

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MCM2 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F4D10

Kat-Nr. : 66204-1-PBS

Synonyme

1F4D10, BM28, CCNL1, CDCL1, EC:3.6.4.12

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  WB analysis using 66204-1-Ig (same clone as 66204-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66204-1-Ig (same clone as 66204-1-PBS)

  WB result of MCM2 antibody (66204-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66204-1-Ig (same clone as 66204-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 66204-1-Ig (same clone as 66204-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66204-1-Ig (same clone as 66204-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66204-1-Ig (same clone as 66204-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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  IHC staining of Human tonsillitis using 66204-1-Ig (same clone as 66204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66204-1-Ig (MCM2 antibody) at dilution of 1:2000 (under 20x lens). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

• 

  IHC staining of Human tonsillitis using 66204-1-Ig (same clone as 66204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66204-1-Ig (MCM2 antibody) at dilution of 1:2000 (under 20x lens). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66204-1-Ig (same clone as 66204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66204-1-Ig (MCM2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66204-1-Ig (same clone as 66204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66204-1-Ig (MCM2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66204-1-PBS bindet in WB, IHC, Indirect ELISA MCM2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MCM2 fusion protein Ag0798
• Vollständiger Name: minichromosome maintenance complex component 2
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 116-125 kDa
• GenBank-Zugangsnummer: BC007670
• Gene symbol: MCM2
• Gene ID (NCBI): 4171
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.