MCM2 Rekombinanter Antikörper

MCM2 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241968F7

Kat-Nr. : 84511-5-PBS

Synonyme

KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28

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  WB analysis using 84511-5-RR (same clone as 84511-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84511-5-RR (MCM2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 84511-5-RR (same clone as 84511-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 84511-5-RR (same clone as 84511-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84511-5-RR (same clone as 84511-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84511-5-RR (same clone as 84511-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84511-5-RR (same clone as 84511-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84511-5-RR (same clone as 84511-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 84511-5-RR (same clone as 84511-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 84511-5-RR (same clone as 84511-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

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  IHC staining of human cervical squamous cancer using 84511-5-RR (same clone as 84511-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

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  IHC staining of human cervical squamous cancer using 84511-5-RR (same clone as 84511-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 84511-5-RR (MCM2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 84511-5-RR (same clone as 84511-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCM2 antibody (84511-5-RR, Clone: 241968F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84511-5-RR (same clone as 84511-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM2 antibody (84511-5-RR, Clone: 241968F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84511-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84511-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84511-5-RR against Human MCM2 were performed. The affinity constant is 2.04 nM.

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Geprüfte Anwendungen

Produktinformation

84511-5-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MCM2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MCM2 fusion protein Ag0798
• Vollständiger Name: minichromosome maintenance complex component 2
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 116-125 kDa
• GenBank-Zugangsnummer: BC007670
• Gene symbol: MCM2
• Gene ID (NCBI): 4171
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.