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MCM2 Polyklonaler Antikörper

MCM2 Polyklonal Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-10513

Synonyme

KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28

Filter:
  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated MCM2 Polyclonal anti (CL488-10513)

    IF Staining of MCF-7 using CL488-10513

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 MCM2 antibody (CL488-10513) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-10513 bindet in IF/ICC MCM2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MCM2 fusion protein Ag0798
Vollständiger Name minichromosome maintenance complex component 2
Berechnetes Molekulargewicht 102 kDa
Beobachtetes Molekulargewicht 116-125 kDa
GenBank-ZugangsnummerBC007670
Gene symbol MCM2
Gene ID (NCBI) 4171
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL Plus 488 MCM2 antibody CL488-10513Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen