MCM5 Polyklonaler Antikörper

MCM5 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11703-1-PBS

Synonyme

CDC46, EC:3.6.4.12, P1 CDC46, P1-CDC46

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  WB analysis using 11703-1-AP (same clone as 11703-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11703-1-AP (MCM5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  WB analysis using 11703-1-AP (same clone as 11703-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11703-1-AP (MCM5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 11703-1-AP (same clone as 11703-1-PBS)

  IP result of anti-MCM5 (IP:11703-1-AP, 4ug; Detection:11703-1-AP 1:4000) with Jurkat cells lysate 2400 ug. This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 11703-1-AP (same clone as 11703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 11703-1-AP (same clone as 11703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 11703-1-AP (same clone as 11703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 11703-1-AP (same clone as 11703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11703-1-AP (same clone as 11703-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM5 antibody (11703-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11703-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA MCM5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MCM5 fusion protein Ag2271
• Vollständiger Name: minichromosome maintenance complex component 5
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 82-88 kDa
• GenBank-Zugangsnummer: BC000142
• Gene symbol: MCM5
• Gene ID (NCBI): 4174
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.