MCM5 Monoklonaler Antikörper

MCM5 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3B7F8

Kat-Nr. : 67049-1-PBS

Synonyme

CDC46, CDC46 homolog, MCM5, P1 CDC46

---

• 

  WB analysis using 67049-1-Ig (same clone as 67049-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67049-1-Ig (MCM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 67049-1-Ig (same clone as 67049-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 67049-1-Ig (MCM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67049-1-Ig (same clone as 67049-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67049-1-Ig (MCM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67049-1-Ig (same clone as 67049-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67049-1-Ig (MCM5 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67049-1-PBS bindet in WB, IF/ICC, Indirect ELISA MCM5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MCM5 fusion protein Ag28704
• Vollständiger Name: minichromosome maintenance complex component 5
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 82-88 kDa
• GenBank-Zugangsnummer: BC000142
• Gene symbol: MCM5
• Gene ID (NCBI): 4174
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.