MCOLN3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MCOLN3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 13879-1-AP

Synonyme

Mucolipin 3, Transient receptor potential channel mucolipin 3, TRP ML3, TRPML3

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  WB analysis using 13879-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13879-1-AP (Mucolipin 3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HeLa-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13879-1-AP bindet in WB, IF, CoIP, ELISA MCOLN3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MCOLN3 fusion protein Ag4962
• Vollständiger Name: mucolipin 3
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC060765
• Gene symbol: MCOLN3
• Gene ID (NCBI): 55283
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCOLN3 (Mucolipin 3) is a member of the TRPML (Transient Receptor Potential Mucolipin) gene family and encodes a non-selective cation channel protein that is primarily localized on the membranes of endosomes and lysosomes. MCOLN3 promotes endosome acidification, regulates membrane transport and fusion, and is involved in the autophagy process. Additionally, MCOLN3 is involved in the regulation of intracellular calcium (Ca²⁺) and iron (Fe²⁺) ion homeostasis, maintains lysosomal membrane potential, promotes endosome-lysosome fusion and material transport (such as autophagy), and may also be involved in neuronal signaling and immune regulation.

Publikationen

Species	Application	Title
human	WB	Traffic; The Ca(2+) channel TRPML3 regulates membrane trafficking and autophagy.; Authors - Hyun Jin Kim ; KD Validated
mouse,human	WB,IF,CoIP	Cell Mol Gastroenterol Hepatol; FGL2-MCOLN3-Autophagy Axis-Triggered Neutrophil Extracellular Traps Exacerbate Liver Injury in Fulminant Viral Hepatitis; Authors - Xitang Li
human	WB	Phytomedicine; Baicalin induces cell death of non-small cell lung cancer cells via MCOLN3-mediated lysosomal dysfunction and autophagy blockage; Authors - Xian Dong ; KD Validated