MCP-1/CCL2 Rekombinanter Antikörper

MCP-1/CCL2 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241165G2

Kat-Nr. : 80847-7-PBS

Synonyme

CCL2, MCP1, MCP-1, C C motif chemokine 2, C-C motif chemokine 2

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  Sandwich ELISA sample test of MP00938-1

  Urine of eight individual healthy human donors was measured. The human MCP-1 concentration of detected samples was determined to be 385.1 pg/mL with a range of 106.1 - 999.8 pg/mL.

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  Sandwich ELISA sample test of MP00938-1

  Human peripheral blood mononuclear cells (1 x 10^6 cells/mL) were cultured in RPMI-1640 supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates were removed on days 3 and 5 and assayed for levels of human MCP-1.

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  Sandwich ELISA sample test of MP00938-1

  Serum of sixteen individual healthy human donors was measured. The human MCP-1 concentration of detected samples was determined to be 595.9 pg/mL with a range of 193.4 - 1,105.3 pg/mL.

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  Sandwich ELISA standard curve of MP00938-1

  Sandwich ELISA standard curve of MP00938-1, Human MCP-1 Recombinant Matched Antibody Pair - PBS only. 80847-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg31375. 80847-7-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL

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  Cytometric bead array standard curve of MP00938-1

  Cytometric bead array standard curve of MP00938-1, MCP-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80847-4-PBS. Detection antibody: 80847-7-PBS. Standard: Eg31375. Range: 0.313-20 ng/mL.

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Geprüfte Anwendungen

Produktinformation

80847-7-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA MCP-1/CCL2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MCP-1/CCL2 fusion protein Eg31375
• Vollständiger Name: chemokine (C-C motif) ligand 2
• Berechnetes Molekulargewicht: 99 aa, 11 kDa
• GenBank-Zugangsnummer: BC009716
• Gene symbol: MCP-1/CCL2
• Gene ID (NCBI): 6347
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.