MCRS1 Polyklonaler Antikörper

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MCRS1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11362-1-PBS

Synonyme

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  WB analysis of Jurkat using 11362-1-AP (same clone as 11362-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11362-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 11362-1-AP (same clone as 11362-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11362-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse uterus using 11362-1-AP (same clone as 11362-1-PBS)

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11362-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 11362-1-AP (same clone as 11362-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11362-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 11362-1-AP (same clone as 11362-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11362-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 11362-1-AP (same clone as 11362-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11362-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 11362-1-AP (same clone as 11362-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11362-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat spleen using 11362-1-AP (same clone as 11362-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11362-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 11362-1-AP (same clone as 11362-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11362-1-AP (MCRS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11362-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11362-1-PBS bindet in WB, IHC, Indirect ELISA MCRS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MCRS1 fusion protein Ag1904
• Vollständiger Name: microspherule protein 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC011794
• Gene symbol: MCRS1
• Gene ID (NCBI): 10445
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCRS1, also named INO80Q, MSP58 or p78, is a nucleolar protein expressed in the very early S phase. It modulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. MCRS1 is also present on polyribosomes of synaptoneurosome as a novel RNA-binding protein to activate the rRNA transcription. The forkhead-associated domain of MCRS1 is involved in association with centrosomal protein Nde1, comprising a component of the centrosome and taking an essential role in viability. Its isoform MCRS2 might be a linker between telomere maintenance and cell-cycle regulation by interacting with LPTS/PinX1, a telomerase-inhibitory protein. MCRS1 is detected by Western blot as a highly modified protein at 78 kDa and minor bands at about 62 and 55 kDa.