MCT4 Rekombinanter Antikörper

MCT4 Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242296E3

Kat-Nr. : 84797-4-PBS

Synonyme

MCT-4, SLC16A3, 242296E3, MCT 4, MCT3

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  WB analysis using 84797-4-RR (same clone as 84797-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84797-4-RR (MCT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84797-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84797-4-RR (same clone as 84797-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84797-4-RR (MCT4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84797-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84797-4-RR (same clone as 84797-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84797-4-RR (MCT4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84797-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 84797-4-RR (same clone as 84797-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MCT4 antibody (84797-4-RR, Clone: 242296E3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84797-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 84797-4-RR (same clone as 84797-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MCT4 antibody (84797-4-RR, Clone: 242296E3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84797-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84797-4-RR (same clone as 84797-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT4 antibody (84797-4-RR, Clone: 242296E3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84797-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84797-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84797-4-RR against Human MCT4 were performed. The affinity constant is 3.02 nM.

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Geprüfte Anwendungen

Produktinformation

84797-4-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA MCT4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MCT4 fusion protein Ag18788
• Vollständiger Name: solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
• Berechnetes Molekulargewicht: 465 aa, 49 kDa
• Beobachtetes Molekulargewicht: 38-42 kDa
• GenBank-Zugangsnummer: BC112269
• Gene symbol: MCT4
• Gene ID (NCBI): 9123
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The monocarboxylate transporter 4 (MCT4, also known as SLC16A3) is involved in the transportation of metabolically important monocarboxylates such as lactate, pyruvate, acetate and ketone bodies. It is widely expressed, particularly strongly in glycolytic tissues such as white skeletal muscle fibres, astrocytes, white blood cells, chondrocytes and some mammalian cell lines. MCT4 is also linked to tumor biology because it mediates lactate transport across membranes resulting in antiapoptotic effects.