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MCU/CCDC109A Rekombinanter Antikörper

MCU/CCDC109A Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250879C3

Kat-Nr. : 86274-1-PBS

Synonyme

CCDC109A, MCU, C10orf42, Calcium uniporter protein, Calcium uniporter protein, mitochondrial

Filter:
  • Western Blot (WB) analysis of various lysates using MCU/CCDC109A Recombinant antibody (86274-1-RR)

    WB analysis using 86274-1-RR (same clone as 86274-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86274-1-RR (CCDC109A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86274-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86274-1-RR

    Affinity and Kinetic Characterization of 86274-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86274-1-RR against Human MCU/CCDC109A were performed. The affinity constant is 1.57 nM.

Geprüfte Anwendungen

Produktinformation

86274-1-PBS bindet in WB, Indirect ELISA MCU/CCDC109A und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen MCU/CCDC109A fusion protein Ag24733
Vollständiger Name coiled-coil domain containing 109A
Berechnetes Molekulargewicht 35 kDa
Beobachtetes Molekulargewicht 30-35 kDa
GenBank-ZugangsnummerBC034235
Gene symbol CCDC109A
Gene ID (NCBI) 90550
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCU, also known as CCDC109A, is highly conserved across all eukaryotes. The MCU protein is composed of two coiled-coil domains, two TMDs, and a short motif of amino acids between the two TMDs. Knockdown of MCU dramatically reduces mitochondrial Ca2+ uptake in isolated mitochondria, in permeabilized cells and living cells. MCU has 3 isoforms with MW 40,37 and 35 kDa (refer to UniProt). The observed MW of MCU is mainly between 30 to 35 kDa in paper (PMID: 26341627; 28337252).