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MDC1 Polyklonaler Antikörper

MDC1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24721-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using MDC1 Polyclonal antibody (24721-1-AP)

    WB analysis using 24721-1-AP (same clone as 24721-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24721-1-AP (MDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MDC1 Polyclonal antibody (24721-1-AP)

    IHC staining of mouse testis using 24721-1-AP (same clone as 24721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24721-1-AP (MDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MDC1 Polyclonal antibody (24721-1-AP)

    IHC staining of mouse testis using 24721-1-AP (same clone as 24721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24721-1-AP (MDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24721-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MDC1 Polyclonal antibody (24721-1-AP)

    IF Staining of HEK-293 using 24721-1-AP (same clone as 24721-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MDC1 antibody (24721-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 24721-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

24721-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MDC1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MDC1 fusion protein Ag20135
Vollständiger Name mediator of DNA damage checkpoint 1
Berechnetes Molekulargewicht 2089 aa, 227 kDa
GenBank-ZugangsnummerBC152556
Gene symbol MDC1
Gene ID (NCBI) 9656
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.