MDC1 Rekombinanter Antikörper

MDC1 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240469D5

Kat-Nr. : 83496-1-PBS

Synonyme

240469D5, MDC 1, NFBD 1, NFBD1

---

• 

  WB analysis of human testis using 83496-1-RR (same clone as 83496-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 83496-1-RR (MDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83496-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 83496-1-RR (same clone as 83496-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83496-1-RR (MDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83496-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00487-1

  Sandwich ELISA standard curve of MP00487-1, Human MDC1 Recombinant Matched Antibody Pair - PBS only. 83496-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag20135. 83496-1-PBS was HRP conjugated as the detection antibody. Range: 39.06-2500 pg/mL

• 

  Sample test of MP00487-1

  L02 and Hela cell lysate were measured. The human MDC1 concentration of detected samples was determined to be 42.2 ng/mL (based on a 2.6 mg/mL extract load) in L02 cell lysate and 7.6 ng/mL (based on a 1.2 mg/mL extract load) in Hela cell lysate.

• 

  IF Staining of HeLa using 83496-1-RR (same clone as 83496-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MDC1 antibody (83496-1-RR, Clone: 240469D5 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83496-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00487-1

  Cytometric bead array standard curve of MP00487-1, MDC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83496-5-PBS. Detection antibody: 83496-1-PBS. Standard: Ag20135. Range: 0.313-40 ng/mL.

• 

  Affinity and Kinetic Characterization of 83496-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83496-1-RR against Human MDC1 were performed. The affinity constant is 0.346 nM.

---

Geprüfte Anwendungen

Produktinformation

83496-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test MDC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MDC1 fusion protein Ag20135
• Vollständiger Name: mediator of DNA damage checkpoint 1
• Berechnetes Molekulargewicht: 2089 aa, 227 kDa
• GenBank-Zugangsnummer: BC152556
• Gene symbol: MDC1
• Gene ID (NCBI): 9656
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.