MDH1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

MDH1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F9A2

Kat-Nr. : 66505-1-PBS

Synonyme

MDHA, KAR, EC:1.1.1.96, EC:1.1.1.37, Aromatic alpha-keto acid reductase

---

• 

  WB analysis using 66505-1-Ig (same clone as 66505-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66505-1-Ig (MDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66505-1-Ig (same clone as 66505-1-PBS)

  WB result of MDH1 antibody (66505-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDH1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66505-1-Ig (same clone as 66505-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66505-1-Ig (MDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66505-1-Ig (same clone as 66505-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66505-1-Ig (MDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 66505-1-Ig (same clone as 66505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66505-1-Ig (MDH1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 66505-1-Ig (same clone as 66505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66505-1-Ig (MDH1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66505-1-Ig (same clone as 66505-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66505-1-Ig (MDH1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 66505-1-Ig (same clone as 66505-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human MDH1 (66505-1-Ig, Clone:1F9A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66505-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66505-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA MDH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MDH1 fusion protein Ag8744
• Vollständiger Name: malate dehydrogenase 1, NAD (soluble)
• Berechnetes Molekulargewicht: 334 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36-37 kDa
• GenBank-Zugangsnummer: BC001484
• Gene symbol: MDH1
• Gene ID (NCBI): 4190
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme. (PMID:10693743)