MDH1 Rekombinanter Antikörper

MDH1 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241887A1

Kat-Nr. : 84576-4-PBS

Synonyme

MDHA, KAR, EC:1.1.1.96, EC:1.1.1.37, Aromatic alpha-keto acid reductase

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  WB analysis using 84576-4-RR (same clone as 84576-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84576-4-RR (MDH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84576-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84576-4-RR (same clone as 84576-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84576-4-RR (MDH1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84576-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84576-4-RR (same clone as 84576-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84576-4-RR (MDH1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84576-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84576-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84576-4-RR against Human MDH1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84576-4-PBS bindet in WB, IHC, Indirect ELISA MDH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MDH1 fusion protein Ag8744
• Vollständiger Name: malate dehydrogenase 1, NAD (soluble)
• Berechnetes Molekulargewicht: 334 aa, 36 kDa
• Beobachtetes Molekulargewicht: 35-37 kDa
• GenBank-Zugangsnummer: BC001484
• Gene symbol: MDH1
• Gene ID (NCBI): 4190
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme.(PMID:10693743)