MDM1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MDM1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 17575-1-AP

Synonyme

Nuclear protein MDM1

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  WB analysis using 17575-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17575-1-AP (MDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17575-1-AP

  WB result of MDM1 antibody (17575-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDM1 transfected HEK-293 cells.

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  WB analysis of Raji using 17575-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 17575-1-AP (MDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 17575-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17575-1-AP (MDM1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17575-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17575-1-AP (MDM1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of mouse eye using 17575-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using MDM1 antibody (17575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

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  IF Staining of mouse eye using 17575-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using MDM1 antibody (17575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red).

• 

  IF Staining of mouse eye using 17575-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using MDM1 antibody (17575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 using 17575-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using MDM1 antibody (17575-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

• 

  IF Staining of hTERT-RPE1 using 17575-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using MDM1 antibody (17575-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Retina-Gewebe, HEK-293-Zellen, Raji-Zellen, Ratten-Retina-Gewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Augengewebe
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IP: See 1 publications below

Produktinformation

17575-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA MDM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MDM1 fusion protein Ag10514
• Vollständiger Name: Mdm1 nuclear protein homolog (mouse)
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: BC028355
• Gene symbol: MDM1
• Gene ID (NCBI): 56890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mdm1 was upregulated during ciliogenesis in multiciliated tracheal epithelial cells and localized to centrioles and cilia when transfected into NIH/3T3 cells. Mdm1 is a microtubule-binding protein that inhibits centrosome duplication when overexpressed. Proteomic analysis revealed that Mdm1 was also detected in the mouse photoreceptor sensory cilium complex and the outer segment (OS) of photoreceptor cells in the retina, suggesting that Mdm1 might be involved in the regulation of the functions and structure of the centrosome, as well as cilia. (PMID: 36171205)

Publikationen

Species	Application	Title
human	WB,IP	Cancer Biol Med; MDM1 overexpression promotes p53 expression and cell apoptosis to enhance therapeutic sensitivity to chemoradiotherapy in patients with colorectal cancer; Authors - Ningxin Ren
mouse	WB	Adv Sci (Weinh); m6A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional Control; Authors - Lisha Yin
mouse	WB	Cell Death Dis; Mdm1 ablation results in retinal degeneration by specific intraflagellar transport defects of photoreceptor cells; Authors - Youlim Son ; KO Validated