MDM1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

MDM1 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

230425E10

Kat-Nr. : 83141-2-PBS

Synonyme

230425E10, Nuclear protein MDM1

---

• 

  WB analysis using 83141-2-RR (same clone as 83141-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83141-2-RR (MDM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83141-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 83141-2-RR (same clone as 83141-2-PBS)

  WB result of MDM1 antibody (83141-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDM1 transfected HEK-293 cells. This data was developed using the same antibody clone with 83141-2-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00166-1

  Sandwich ELISA standard curve of MP00166-1, Human MDM1 Recombinant Matched Antibody Pair - PBS only. 83141-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10541. 83141-2-PBS was HRP conjugated as the detection antibody. Range: 46.8-3000 pg/mL.

• 

  Sample test of MP00166-1

  The mean MDM1 concentration was determined to be 1,326.4 pg/mL in Raji cell extract based on a 4.0 mg/mL extract load.

• 

  IHC staining of mouse testis using 83141-2-RR (same clone as 83141-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83141-2-RR (MDM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83141-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00166-1

  Cytometric bead array standard curve of MP00166-1, MDM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83141-3-PBS. Detection antibody: 83141-2-PBS. Standard: Ag10541. Range: 0.625-20 ng/mL.

• 

  Affinity and Kinetic Characterization of 83141-2-PBS

  Biolayer interferometry (BLl) kinetic assays of 83141-2-PBS against Human MDM1 were performed. The affinity constant is 3.50 nM.

• 

  Affinity and Kinetic Characterization of 83141-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83141-2-RR against Human MDM1 were performed. The affinity constant is 3.50 nM.

---

Geprüfte Anwendungen

Produktinformation

83141-2-PBS bindet in WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test MDM1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MDM1 fusion protein Ag10541
• Vollständiger Name: Mdm1 nuclear protein homolog (mouse)
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 102 kDa
• GenBank-Zugangsnummer: BC028355
• Gene symbol: MDM1
• Gene ID (NCBI): 56890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mdm1 was upregulated during ciliogenesis in multiciliated tracheal epithelial cells and localized to centrioles and cilia when transfected into NIH/3T3 cells. Mdm1 is a microtubule-binding protein that inhibits centrosome duplication when overexpressed. Proteomic analysis revealed that Mdm1 was also detected in the mouse photoreceptor sensory cilium complex and the outer segment (OS) of photoreceptor cells in the retina, suggesting that Mdm1 might be involved in the regulation of the functions and structure of the centrosome, as well as cilia. (PMID: 36171205)