MDM1 Rekombinanter Antikörper

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• KD/KO Validated

MDM1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230425E10

Kat-Nr. : 83141-2-RR

Synonyme

Nuclear protein MDM1, 230425E10

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• 

  WB analysis using 83141-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83141-2-RR (MDM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 83141-2-RR

  WB result of MDM1 antibody (83141-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDM1 transfected HEK-293 cells.

• 

  IHC staining of mouse testis using 83141-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83141-2-RR (MDM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83141-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83141-2-RR against Human MDM1 were performed. The affinity constant is 3.50 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maushodengewebe, PC-3-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83141-2-RR bindet in WB, IHC, ELISA MDM1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MDM1 fusion protein Ag10541
• Vollständiger Name: Mdm1 nuclear protein homolog (mouse)
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 102 kDa
• GenBank-Zugangsnummer: BC028355
• Gene symbol: MDM1
• Gene ID (NCBI): 56890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mdm1 was upregulated during ciliogenesis in multiciliated tracheal epithelial cells and localized to centrioles and cilia when transfected into NIH/3T3 cells. Mdm1 is a microtubule-binding protein that inhibits centrosome duplication when overexpressed. Proteomic analysis revealed that Mdm1 was also detected in the mouse photoreceptor sensory cilium complex and the outer segment (OS) of photoreceptor cells in the retina, suggesting that Mdm1 might be involved in the regulation of the functions and structure of the centrosome, as well as cilia. (PMID: 36171205)