MDMX/MDM4 Polyklonaler Antikörper

MDMX/MDM4 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 28747-1-AP

Synonyme

MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX

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  WB analysis using 28747-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28747-1-AP (MDMX antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28747-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28747-1-AP (MDMX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 28747-1-AP

  IP result of anti-MDMX (IP:28747-1-AP, 4ug; Detection:28747-1-AP 1:3000) with MCF-7 cells lysate 1470 ug.

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  IHC staining of human ovary tumor using 28747-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28747-1-AP (MDM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28747-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28747-1-AP (MDM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 28747-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28747-1-AP (MDMX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen, U2OS-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

28747-1-AP bindet in WB, IHC, IP, ELISA MDMX/MDM4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MDMX/MDM4 fusion protein Ag30456
• Vollständiger Name: Mdm4 p53 binding protein homolog (mouse)
• Berechnetes Molekulargewicht: 490 aa, 55 kDa
• Beobachtetes Molekulargewicht: 70-76 kDa
• GenBank-Zugangsnummer: BC067299
• Gene symbol: MDMX/MDM4
• Gene ID (NCBI): 4194
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kDa, while the observed mass is 70-80 kDa, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370, 28097652). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Cancer Sci; SNRPB2 promotes triple-negative breast cancer progression by controlling alternative splicing of MDM4 pre-mRNA; Authors - Shiyi Yu
human	WB	J Med Chem; Design of Murine Double Minute 2 Proteolysis Targeting Chimera Degraders with a Built-In Tumor-Targeting Ability; Authors - Zhuqian Wang