MDMX/MDM4 Rekombinanter Antikörper

MDMX/MDM4 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241936D11

Kat-Nr. : 84534-5-RR

Synonyme

MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX

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  WB analysis using 84534-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 84534-5-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 84534-5-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84534-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84534-5-RR against Human MDMX/MDM4 were performed. The affinity constant is 0.247 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A549-Zellen, HEK-293T-Zellen, MCF-7-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84534-5-RR bindet in WB, IHC, ELISA MDMX/MDM4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MDMX/MDM4 fusion protein Ag26678
• Vollständiger Name: Mdm4 p53 binding protein homolog (mouse)
• Berechnetes Molekulargewicht: 490 aa, 55 kDa
• Beobachtetes Molekulargewicht: 70-76 kDa
• GenBank-Zugangsnummer: BC067299
• Gene symbol: MDMX/MDM4
• Gene ID (NCBI): 4194
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kDa, while the observed mass is 70-80 kDa, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370, 28097652). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.