ME1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ME1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16619-1-PBS

Synonyme

EC:1.1.1.40, Malic enzyme 1, NADP-dependent malic enzyme, NADP-ME

---

• 

  WB analysis using 16619-1-AP (same clone as 16619-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse placenta using 16619-1-AP (same clone as 16619-1-PBS)

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 16619-1-AP (same clone as 16619-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 16619-1-AP (same clone as 16619-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 16619-1-AP (same clone as 16619-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse liver using 16619-1-AP (same clone as 16619-1-PBS)

  IP result of anti-ME1 (IP:16619-1-AP, 4ug; Detection:16619-1-AP 1:2000) with mouse liver tissue lysate 2400 ug. This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16619-1-AP (same clone as 16619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16619-1-AP (same clone as 16619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16619-1-AP (same clone as 16619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16619-1-AP (same clone as 16619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 16619-1-AP (same clone as 16619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 16619-1-AP (same clone as 16619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 16619-1-AP (same clone as 16619-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ME1 antibody (16619-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16619-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

16619-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA ME1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ME1 fusion protein Ag9916
• Vollständiger Name: malic enzyme 1, NADP(+)-dependent, cytosolic
• Berechnetes Molekulargewicht: 572 aa, 64 kDa
• Beobachtetes Molekulargewicht: 55-64 kDa
• GenBank-Zugangsnummer: BC025246
• Gene symbol: ME1
• Gene ID (NCBI): 4199
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ME1(NADP-dependent malic enzyme) belongs to the malic enzymes family. This malic enzyme catalyzes the reversible oxidative decarboxylation of malate and is a link between the glycolytic pathway and the citric acid cycle. The reaction is L-malate plus NADP(+) to form pyruvate, CO(2), and NADPH. The predicted protein contains 572 amino acids and has a calculated molecular mass of 64.1 kDa.