ME2 Polyklonaler Antikörper

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• KD/KO Validated

ME2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 24944-1-AP

Synonyme

Malic enzyme 2, ME2, NAD ME

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 24944-1-AP

  WB result of ME2 antibody (24944-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ME2 transfected HepG2 cells.

• 

  WB analysis using 24944-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24944-1-AP (ME2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24944-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24944-1-AP (ME2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24944-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24944-1-AP (ME2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24944-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24944-1-AP (ME2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 24944-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 24944-1-AP (ME2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24944-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24944-1-AP (ME2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24944-1-AP

  IP Result of anti-ME2 (IP:24944-1-AP, 4ug; Detection:24944-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human pancreas cancer using 24944-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24944-1-AP (ME2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 24944-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24944-1-AP (ME2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 24944-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24944-1-AP (ME2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 24944-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24944-1-AP (ME2 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

24944-1-AP bindet in WB, IP, IHC, ELISA ME2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ME2 fusion protein Ag20741
• Vollständiger Name: malic enzyme 2, NAD(+)-dependent, mitochondrial
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 54 kDa, 65 kDa
• GenBank-Zugangsnummer: BC000147
• Gene symbol: ME2
• Gene ID (NCBI): 4200
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ME2(malic enzyme 2) is also named as NAD-ME and belongs to the malic enzymes family. ME1, ME2 are important for NADPH production, lipogenesis and glutamine metabolism, but ME2 has a more profound effect. ME2 is a genome-coded mitochondrial enzyme that converts malate to pyruvate and is involved in neuronal synthesis of the neurotransmitter γ-aminobutyric acid (GABA)(PMID:15532013). This protein has 2 isoforms produced by alternative splicing with the MW of 65 kDa and 54 kDa.

Publikationen

Species	Application	Title
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
human	WB	Adv Sci (Weinh); SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma; Authors - Qifan Hu
human	WB	Cell Death Differ; SIRT5-mediated ME2 desuccinylation promotes cancer growth by enhancing mitochondrial respiration; Authors - Peng Teng ; KD Validated