MECR Polyklonaler Antikörper

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MECR Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 51025-2-PBS

Synonyme

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  WB analysis of mouse skeletal muscle using 51025-2-Ig (same clone as 51025-2-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 51025-2-Ig (MECR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51025-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 51025-2-Ig (same clone as 51025-2-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 51025-2-Ig (MECR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51025-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 51025-2-Ig (same clone as 51025-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 51025-2-Ig (MECR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51025-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 51025-2-Ig (same clone as 51025-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 51025-2-Ig (MECR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51025-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

51025-2-PBS bindet in WB, IHC, Indirect ELISA MECR und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitochondrial trans-2-enoyl-CoA reductase
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC003864
• Gene symbol: MECR
• Gene ID (NCBI): 26922
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitochondrial Trans-2-Enoyl-CoA Reductase is abbrivated as MECR, involved in human mitochonrial fatty acid synthesis(mtFAS) as an oxidoreductase. (PMID: 27817865) MECR has molecular weight can be calculated around 37KDa. With immunopercipitation reaction on mouse heart samples, the IgG heavy chain, dimer of MECR is around 65KDa. Numerous studies have shown disorder of lipid metabolism is closely related with malignance, especially in liver cancer. As futher research suggests that MECR has close realitionship with cell prolifereation, appotosis, and metastasis (PMID: 31178528)