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MED10 Polyklonaler Antikörper

MED10 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15196-1-AP

Synonyme

L6, MED10, mediator complex subunit 10, NUT2, TRG 17, TRG 20, TRG17, TRG20

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of COLO 320 cells using MED10 Polyclonal antibody (15196-1-AP)

    WB analysis of COLO 320 using 15196-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 15196-1-AP (MED10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBCOLO 320-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

15196-1-AP bindet in WB,ELISA MED10 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MED10 fusion protein Ag7336
Vollständiger Name mediator complex subunit 10
Berechnetes Molekulargewicht 16 kDa
Beobachtetes Molekulargewicht 16 kDa
GenBank-ZugangsnummerBC003353
Gene symbol MED10
Gene ID (NCBI) 84246
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MED10, also named as L6, TRG17, or TRG20, is a 135 amino acid protein, which belongs to the Mediator complex subunit 10 family. MED10 localizes in the nucleus and as a component of the Mediator complex, a coactivator is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Multiprotein mediator complex acting as a coactivator is required for activation of RNA polymerase II transcription by DNA bound transcription factors.

Protokolle

Produktspezifische Protokolle
WB protocol for MED10 antibody 15196-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

J Virol

Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex.

Authors - Vijayalingam S S
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

SITING (Verified Customer) (04-02-2021)

can see band aroun 15kd

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: EMBRYO HEART
MED10 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:15196-1-AP)