MED15 Polyklonaler Antikörper

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MED15 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11566-1-PBS

Synonyme

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  WB analysis of K-562 using 11566-1-AP (same clone as 11566-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SMMC-7721 using 11566-1-AP (same clone as 11566-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HuH-7 using 11566-1-AP (same clone as 11566-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 11566-1-AP (same clone as 11566-1-PBS)

  IP result of anti-MED15 (IP:11566-1-AP, 3ug; Detection:11566-1-AP 1:500) with K-562 cells lysate 3440ug. This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11566-1-AP (same clone as 11566-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11566-1-AP (MED15 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 11566-1-AP (same clone as 11566-1-PBS)

  Immunofluorescent analysis of SH-SY5Y cells using 11566-1-AP (MED15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11566-1-PBS bindet in WB, IHC, IF/ICC, IP, ELISA MED15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MED15 fusion protein Ag2143
• Vollständiger Name: mediator complex subunit 15
• Berechnetes Molekulargewicht: 788 aa, 87 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC017110
• Gene symbol: MED15
• Gene ID (NCBI): 51586
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MED15, also named as TIG1, CAG7A, CTG7A, PCQAP, TIG-1, TNRC7 and ARC105, is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. It contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.MED15 is recruited to promoters by direct interaction with gene-specific regulatory proteins. It is about 105kd in SDS-page(doi.org/10.3109/10409238.2013.840259).