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MED18 Monoklonaler Antikörper

MED18 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D10G8

Kat-Nr. : 60467-1-PBS

Synonyme

2D10G8, Mediator complex subunit 18, p28b

Filter:
  • Western Blot (WB) analysis of various lysates using MED18 Monoclonal antibody (60467-1-Ig)

    WB analysis using 60467-1-Ig (same clone as 60467-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60467-1-Ig (MED18 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60467-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MED18 Monoclonal antibody (60467-1-Ig)

    IF Staining of A431 using 60467-1-Ig (same clone as 60467-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MED18 antibody (60467-1-Ig, Clone: 2D10G8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60467-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

60467-1-PBS bindet in WB, IF/ICC, Indirect ELISA MED18 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen MED18 fusion protein Ag7495
Vollständiger Name mediator complex subunit 18
Berechnetes Molekulargewicht 24 kDa
Beobachtetes Molekulargewicht 24 kDa
GenBank-ZugangsnummerBC002694
Gene symbol MED18
Gene ID (NCBI) 54797
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MED18, also named as Mediator of RNA polymerase II transcription subunit 18, is a 208 amino acid protein, which belongs to the Mediator complex subunit 18 family. MED18 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED18 as a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED18 is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.