MED19 Polyklonaler Antikörper

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• KD/KO Validated

MED19 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 13418-1-AP

Synonyme

DT2P1G7, LCMR1, MED19, mediator complex subunit 19

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  WB analysis of human testis using 13418-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13418-1-AP (MED19 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 13418-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13418-1-AP (MED19 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13418-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13418-1-AP (MED19 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

13418-1-AP bindet in WB, IHC, ELISA MED19 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MED19 fusion protein Ag4165
• Vollständiger Name: mediator complex subunit 19
• Berechnetes Molekulargewicht: 194 aa, 20 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC037223
• Gene symbol: MED19
• Gene ID (NCBI): 219541
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mediator of RNA polymerase II transcription subunit 19 (MED19) is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (PMID: 12584197). Mediator complex is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors (PMID: 15175163). MED19 may be a novel proliferation regulator that promotes lung cancer and gastric cancer progression and cellular growth (PMID: 21519921, PMID: 22565189).

Publikationen

Species	Application	Title
human	WB	J Cell Biochem; Med19 is involved in chemoresistance by mediating autophagy through HMGB1 in breast cancer.; Authors - Beibei Liu ; KD Validated
human	WB	J Biochem Mol Toxicol; Chloroprocaine antagonizes progression of breast cancer by regulating LINC00494/miR-3619-5p/MED19 axis; Authors - Chen Chen
rat	IHC,WB	Int J Nanomedicine; Hypoxic Bone Mesenchymal Stem Cell-Derived Exosomes Direct Schwann Cells Proliferation, Migration, and Paracrine to Accelerate Facial Nerve Regeneration via circRNA_Nkd2/miR-214-3p/MED19 Axis; Authors - Haopeng Wang
		Mol Cell; The phosphatase PP1 sustains global transcription by promoting RNA polymerase II pause release; Authors - Zhenning Wang