MED28 Polyklonaler Antikörper

MED28 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16262-1-AP

Synonyme

1500003D12Rik, EG1, Endothelial derived protein 1, magicin, MED28, mediator complex subunit 28, Tumor angiogenesis marker EG 1

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Galerie der Validierungsdaten

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  WB analysis using 16262-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16262-1-AP (MED28 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 16262-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 16262-1-AP (MED28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, PC-12-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Eierstockgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Lebergewebe, humanes Nierengewebe, humanes Plazenta-Gewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16262-1-AP bindet in WB, IHC,ELISA MED28 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MED28 fusion protein Ag9327
• Vollständiger Name: mediator complex subunit 28
• Berechnetes Molekulargewicht: 178 aa, 20 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC011936
• Gene symbol: MED28
• Gene ID (NCBI): 80306
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.