MED29 Polyklonaler Antikörper

MED29 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 33366-1-PBS

Synonyme

Intersex-like protein, IXL, Mediator complex subunit 29, Mediator of RNA polymerase II transcription subunit 29

---

• 

  WB analysis using 33366-1-AP (same clone as 33366-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 33366-1-AP (MED29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33366-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HT-29 using 33366-1-AP (same clone as 33366-1-PBS)

  IP result of anti-MED29 (IP:33366-1-AP, 4ug; Detection:33366-1-AP 1:800) with HT-29 cells lysate 1760 ug. This data was developed using the same antibody clone with 33366-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 33366-1-AP (same clone as 33366-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 33366-1-AP (MED29 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33366-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

33366-1-PBS bindet in WB, IHC, IP, Indirect ELISA MED29 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MED29 fusion protein Ag38949
• Vollständiger Name: mediator complex subunit 29
• Berechnetes Molekulargewicht: 21kDa，200aa
• Beobachtetes Molekulargewicht: 21-25 kDa
• GenBank-Zugangsnummer: NM_017592.2
• Gene symbol: MED29
• Gene ID (NCBI): 55588
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mediator of RNA polymerase II transcription subunit 29 (MED29) is also named as Mediator complex subunit 29 and Intersex-like protein (IXL). MED29 is one of the most highly conserved proteins across species and is widely expressed in humans both during embryonic development and in adult tissues (PMID: 15555573). MED29 was overexpressed in pancreatic cancer and promoted pancreatic cancer cell viability (PMID: 21225629, PMID: 17332321). MED29 facilitated the epithelial-mesenchymal transition process in OSCC, thereby promoting migration and invasion (PMID: 39462350).