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MED31 Polyklonaler Antikörper

MED31 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16590-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of mouse testis tissue using MED31 Polyclonal antibody (16590-1-AP)

    WB analysis of mouse testis using 16590-1-AP (same clone as 16590-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16590-1-AP (MED31 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16590-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using MED31 Polyclonal antibody (16590-1-AP)

    WB analysis of human brain using 16590-1-AP (same clone as 16590-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 16590-1-AP (MED31 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16590-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MED31 Polyclonal antibody (16590-1-AP)

    IF Staining of HepG2 using 16590-1-AP (same clone as 16590-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MED31 antibody (16590-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16590-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

16590-1-PBS bindet in WB, IF/ICC, Indirect ELISA MED31 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MED31 fusion protein Ag9008
Vollständiger Name mediator complex subunit 31
Berechnetes Molekulargewicht 131 aa, 16 kDa
Beobachtetes Molekulargewicht 16 kDa
GenBank-ZugangsnummerBC012539
Gene symbol MED31
Gene ID (NCBI) 51003
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MED31, also named as SOH1 or CGI-125, is a 131 amino acid protein, which belongs to the Mediator complex subunit 31 family. MED31 localizes in the nucleus. MED31 as a component of the Mediator complex, a coactivator is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED31 functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.