MED6 Rekombinanter Antikörper

MED6 Rekombinant Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241382B2

Kat-Nr. : 84184-5-RR

Synonyme

NY REN 28, Mediator of RNA polymerase II transcription subunit 6, hMed6, ARC33, Activator-recruited cofactor 33 kDa component

---

• 

  IF Staining of A431 using 84184-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MED6 antibody (84184-5-RR, Clone: 241382B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 84184-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84184-5-RR against Human MED6 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84184-5-RR bindet in IF/ICC, ELISA MED6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MED6 fusion protein Ag7541
• Vollständiger Name: mediator complex subunit 6
• Berechnetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC004106
• Gene symbol: MED6
• Gene ID (NCBI): 10001
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.