MED8 Polyklonaler Antikörper

MED8 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12182-1-PBS

Synonyme

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  WB analysis of HepG2 using 12182-1-AP (same clone as 12182-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 12182-1-AP (same clone as 12182-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SGC-7901 using 12182-1-AP (same clone as 12182-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 12182-1-AP (same clone as 12182-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12182-1-AP (MED8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 12182-1-AP (same clone as 12182-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12182-1-AP (MED8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12182-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12182-1-PBS bindet in WB, IHC, Indirect ELISA MED8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MED8 fusion protein Ag2827
• Vollständiger Name: mediator complex subunit 8
• Berechnetes Molekulargewicht: 212 aa, 23 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC010543
• Gene symbol: MED8
• Gene ID (NCBI): 112950
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MED8, a component of the Mediator complex, is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. It plays a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. MED8 has three isoforms with MW 19 kDa, 29 kDa and 33 kDa. And the ubiquitination form will be 38-43 kDa.