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MED9 Polyklonaler Antikörper

MED9 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27452-1-AP

Synonyme

MED25, MED9, mediator complex subunit 9

Filter:
  • Western Blot (WB) analysis of HEK-293T cells using MED9 Polyclonal antibody (27452-1-AP)

    WB analysis of HEK-293T using 27452-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27452-1-AP (MED9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293T-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

27452-1-AP bindet in WB, ELISA MED9 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MED9 fusion protein Ag26158
Vollständiger Name mediator complex subunit 9
Berechnetes Molekulargewicht 146 aa, 16 kDa
Beobachtetes Molekulargewicht 16 kDa
GenBank-ZugangsnummerBC000647
Gene symbol MED9
Gene ID (NCBI) 55090
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MED9, also named as MED25, is a 146 amino acid protein, which belongs to the Mediator complex subunit 9 family. MED9 is a component of the Mediator complex and a coactivator that is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED9 as a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for MED9 antibody 27452-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

siting (Verified Customer) (06-24-2021)

band near to 15kb-10kb

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: mice heart