MEF2C-Specific Polyklonaler Antikörper

MEF2C-Specific Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18293-1-AP

Synonyme

MEF2C, MEF2C-Specific, myocyte enhancer factor 2C

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 18293-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Hirngewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18293-1-AP bindet in WB, IHC, ELISA MEF2C-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myocyte enhancer factor 2C
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 45-70 kDa
• GenBank-Zugangsnummer: NM_002397
• Gene symbol: MEF2C
• Gene ID (NCBI): 4208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. The immunogen used is peptide from the specific sequecne of MEF2C transcript variant 1 match to transcript variant 5. The antibody can recognize the transcript variant 1, transcript variant 2 and transcript variant 3 of MEF2C.