MEF2D Rekombinanter Antikörper

MEF2D Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250068B7

Kat-Nr. : 85801-5-PBS

Synonyme

Myocyte-specific enhancer factor 2D

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  WB analysis using 85801-5-RR (same clone as 85801-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85801-5-RR (MEF2D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 85801-5-RR (same clone as 85801-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85801-5-RR (MEF2D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 85801-5-RR (same clone as 85801-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85801-5-RR (MEF2D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 85801-5-RR (same clone as 85801-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85801-5-RR (MEF2D antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 85801-5-RR (same clone as 85801-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85801-5-RR (MEF2D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 85801-5-RR (same clone as 85801-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEF2D antibody (85801-5-RR, Clone: 250068B7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85801-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85801-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85801-5-RR against Human MEF2D were performed. The affinity constant is 1.99 nM.

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Geprüfte Anwendungen

Produktinformation

85801-5-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MEF2D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MEF2D fusion protein Ag5700
• Vollständiger Name: myocyte enhancer factor 2D
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC040949
• Gene symbol: MEF2D
• Gene ID (NCBI): 4209
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MEF2D, also named as Myocyte-specific enhancer factor 2D, is a 521 amino acid protein, which belongs to the MEF2 family. MEF2D is expressed in t in myotubes and also in undifferentiated myoblasts. MEF2D as a transcriptional activator which binds specifically to the MEF2 element, found in numerous muscle-specific and stress-induced genes. MEF2D mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. The calculated molecular weight of MEF2D is 55 kDa, but Phosphorylated MEF2D is 60 kDa.