MEK1-Specific Polyklonaler Antikörper

MEK1-Specific Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 51080-1-AP

Synonyme

ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, MEK1, MEK1-Specific, MKK1, PRKMK1

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  IHC staining of human endometrial cancer using 51080-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 51080-1-AP (MEK1-Specific antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human endometrial cancer using 51080-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 51080-1-AP (MEK1-Specific antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 51080-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 51080-1-AP (MEK1-Specific antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 51080-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 51080-1-AP (MEK1-Specific antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below

Produktinformation

51080-1-AP bindet in WB, IHC, ELISA MEK1-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Chinesische Wollhandkrabbe (Eriocheir sinensis)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase 1
• Berechnetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC139729
• Gene symbol: MEK1
• Gene ID (NCBI): 5604
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis. MEK1 has 2 isoforms produced by alternative splicing with the molecular weight of 43 kDa and 41 kDa. It can be phosphorylated(PMID:21144847). This antibody is specific to MEK1.

Publikationen

Species	Application	Title
mouse		J Ethnopharmacol; Classical prescription Floris Sophorae Powder treat colorectal cancer by regulating KRAS/MEK-ERK signaling pathway; Authors - Li-Ying Han
human	WB	Cancer Cell Int; MiR-155-5p suppresses SOX1 to promote proliferation of cholangiocarcinoma via RAF/MEK/ERK pathway.; Authors - Da Wang
human	WB	Cancer Cell Int; 4,5-Dimethoxycanthin-6-one is a novel LSD1 inhibitor that inhibits proliferation of glioblastoma cells and induces apoptosis and pyroptosis.; Authors - Wei Li
human	WB	Mol Cell Probes; Elevated PGT promotes proliferation and inhibits cell apoptosis in preeclampsia by Erk signaling pathway; Authors - Huiyuan Pang
human	WB	PLoS One; Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.; Authors - Xu Jiang
human	WB	Onco Targets Ther; FANCI Cooperates with IMPDH2 to Promote Lung Adenocarcinoma Tumor Growth via a MEK/ERK/MMPs Pathway.; Authors - Pengchao Zheng