MEK1 Monoklonaler Antikörper

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• KD/KO Validated

MEK1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3F6D7

Kat-Nr. : 67872-1-Ig

Synonyme

ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MEK 1, MKK1, PRKMK1

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  WB analysis of A431 using 67872-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (MEK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67872-1-Ig

  Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67872-1-Ig

  WB result of MEK1 antibody (67872-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEK1 transfected HeLa cells.

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  WB analysis of NIH/3T3 using 67872-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67872-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 67872-1-Ig

  Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Mit Calyculin A behandelte HeLa-Zellen, Mit Calyculin A behandelte PC-3-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67872-1-Ig bindet in WB, ELISA MEK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase 1
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: BC139729
• Gene symbol: MEK1
• Gene ID (NCBI): 5604
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis.

Publikationen

Species	Application	Title
rat	WB	J Ethnopharmacol; Jia-Wei-Si-Miao-Yong-An Fang stimulates the healing of acute radiation-induced cutaneous wounds through MAPK/ERK pathway; Authors - Yin Wang