MEKK3 Polyklonaler Antikörper

MEKK3 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21072-1-PBS

Synonyme

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  WB analysis of mouse liver using 21072-1-AP (same clone as 21072-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21072-1-AP (MEKK3 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21072-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 21072-1-AP (same clone as 21072-1-PBS)

  IP result of anti-MEKK3 (IP:21072-1-AP, 4ug; Detection:21072-1-AP 1:300) with mouse liver tissue lysate 7000ug. This data was developed using the same antibody clone with 21072-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 21072-1-AP (same clone as 21072-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21072-1-AP (MEKK3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21072-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 21072-1-AP (same clone as 21072-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21072-1-AP (MEKK3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21072-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21072-1-PBS bindet in WB, IHC, IP, Indirect ELISA MEKK3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MEKK3 fusion protein Ag15223
• Vollständiger Name: mitogen-activated protein kinase kinase kinase 3
• Berechnetes Molekulargewicht: 657 aa, 74 kDa
• Beobachtetes Molekulargewicht: 71 kDa, 74 kDa
• GenBank-Zugangsnummer: BC090859
• Gene symbol: MEKK3
• Gene ID (NCBI): 4215
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mitogen-activated protein/ERK kinase kinase 3 (MEKK3) is a member of a family of MEKK/Ste11 serine/threonine protein kinases. One function of MEKK3 is to serve as an upstream regulatory kinase in the mitogen-activated protein kinase cascade, however, it also plays a critical role in other signaling pathways. MEKK3 regulates several important functions including cell survival and proliferation. It is ubiquitously expressed in multiple tissues and has been found in complexes with scaffold proteins and other kinases. (PMID: 18206350).Cerebral cavernous malformations (CCMs) complex regulation of MEKK3 is essential during vertebrate heart development. MEKK3 signalling and KLF2/4 may be targeted to develop new CCM therapeutics.