MELK Rekombinanter Antikörper

MELK Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

230106E8

Kat-Nr. : 82893-6-PBS

Synonyme

EC:2.7.10.2, EC:2.7.11.1, hMELK, hPK38, KIAA0175

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  Sandwich ELISA standard curve of MP00075-4

  Sandwich ELISA standard curve of MP00075-4, Human MELK Recombinant Matched Antibody Pair - PBS only. 82893-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1897. 82893-6-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP00075-4

  NCCIT and K-562 cell lysate were measured. The human MELK concentration of detected samples was determined to be 15.9 ng/mL (based on a 4.5 mg/mL extract load) in NCCIT cell lysate and 16.4 ng/mL (based on a 4.5 mg/mL extract load) in K-562 cell lysate.

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  Affinity and Kinetic Characterization of 82893-6-PBS

  Biolayer interferometry (BLl) kinetic assay of 82893-6-PBS against Human MELK was performed. The affinity constant is 72.3 pM.

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Geprüfte Anwendungen

Produktinformation

82893-6-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test MELK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MELK fusion protein Ag1897
• Vollständiger Name: maternal embryonic leucine zipper kinase
• Berechnetes Molekulargewicht: 651 aa, 75 kDa
• GenBank-Zugangsnummer: BC014039
• Gene symbol: MELK
• Gene ID (NCBI): 9833
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.